The protease activity and R78 cleavage of orthologs from divergent MCDV isolates MCDV-Tn and MCDV-M1, and other waikavirus species including rice tungro spherical virus (RTSV) and bellflower vein chlorosis virus (BVCV) were also examined. Bioinformatics Genomic sequences generated from BAC and PAC clones were used to identify ESTs within dbEST, as well as genes, mRNAs, or cDNAs from the nonredundant (nr) nucleotide database using BLASTn ( http. Mutations that altered the predicted catalytic triad residues and H2817 disrupted MCDV-S protease activity, as did mutagenesis of a conserved tyrosine residue, Y2774. The PCR products were sequenced using the UCSF Biomolecular Resource Center and sequences were assembled using the Macvector program. These and other residues were targeted for mutagenesis and tested for proteolytic cleavage activity on the N-terminal 78 kDa MCDV-S polyprotein substrate to identify mutants that abolished catalytic activity. Page unnumbered Tenth International Conference on AIDS International Conference on STD The Global Challenge of AIDS: Together for the Future PROGRAM Yokohama, Japan 7-12 August 1994 0 0 Page unnumbered This project has been executed with the grant of the Commemorative Association for the Japan World Exposition (1 970). Here, we examined amino acid residues required for catalytic activity of the protease, including those in the predicted catalytic triad, amino acid residues H2667, D2704, and C2798, as well as H2817 hypothesized to be important in substrate binding.
UCSF Box is integrated with MyAccess and offers features such as content and task management, online workspace for collaboration, and user and group permissions. UCSF Box is similar to Dropbox, and GoogleDrive but only UCSF Box and OneDrive are managed and supported by UCSF IT. To identify novel genes required for assembly of the IDAs, we screened several collections of motility mutants in Chlamydomonas for strains that exhibited the slow swimming phenotype typical of ida mutants (Brokaw and Kamiya, 1987). To facilitate genomewide functional analyses, we developed a high-density cell array with quantitative and automated readout. Characterization of new ida mutations and identification of the IDA8/BOP2 locus. Maize chlorotic dwarf virus (MCDV) encodes a 3C-like protease that cleaves the N-terminal polyprotein (R78) as previously demonstrated. Initial attempts where we imaged -secretase in digitonin using an FEI Falcon-II direct-electron detector yielded a 3D reconstruction with a large disc-shaped body and a protruding head, which could accommodate the TMs and extracellular domains of -secretase, respectively (Extended Data Fig. UCSF Box is a cloud-based file collaboration tool. With the advent of complete genome sequences, large-scale functional analyses are generating new excitement in biology and medicine.
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